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Pharmacokinetics of orbifloxacin in crucian carp ( Carassius auratus ) after intravenous and intramuscular administration
Author(s) -
Yang F.,
Yang F.,
Wang G.,
Shi W.,
Kong T.,
Yang P.,
Bai D.,
Zhou B.
Publication year - 2018
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12495
Subject(s) - crucian carp , volume of distribution , pharmacokinetics , bioavailability , chemistry , intramuscular injection , carp , absorption (acoustics) , medicine , pharmacology , biology , fish <actinopterygii> , fishery , physics , acoustics
The pharmacokinetics of orbifloxacin was studied after a single dose (7.5 mg/kg) of intravenous or intramuscular administration to crucian carp ( Carassius auratus ) reared in freshwater at 25°C. Plasma samples were collected from six fish per sampling point. Orbifloxacin concentrations were determined by high‐performance liquid chromatography with a 0.02 μg/ml limit of detection, then were subjected to noncompartmental analysis. After intravenous injection, initial concentration of 5.83 μg/ml, apparent elimination rate constant (λ z ) of 0.039 hr −1 , apparent elimination half‐life (T 1/2λz ) of 17.90 hr, systemic total body clearance (Cl) of 75.47 ml hr −1 kg −1 , volume of distribution (Vz) of 1,948.76 ml/kg, and volume of distribution at steady‐state (Vss) of 1,863.97 ml/kg were determined, respectively. While after intramuscular administration, the λ z , T 1/2λz , mean absorption time ( MAT ), absorption half‐life ( T 1/2ka ), and bioavailability were determined as 0.027 hr −1 , 25.69, 10.26, 7.11 hr, and 96.46%, respectively, while the peak concentration was observed as 3.11 ± 0.06 μg/ml at 2.0 hr. It was shown that orbifloxacin was completely but relatively slowly absorbed, extensively distributed, and slowly eliminated in crucian carp, and an orbifloxacin dosage of 10 mg/kg administered intravenously or intramuscularly would be expected to successfully treat crucian carp infected by strains with MIC values ≤0.5 μg/ml.