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Pharmacokinetics and tissue distribution of enrofloxacin after single intramuscular injection in Pacific white shrimp
Author(s) -
Fang X.,
Zhou J.,
Liu X.
Publication year - 2018
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12431
Subject(s) - enrofloxacin , hemolymph , hepatopancreas , cmax , pharmacokinetics , shrimp , volume of distribution , chemistry , metabolite , ciprofloxacin , pharmacology , biology , biochemistry , antibiotics , fishery
The pharmacokinetic properties and tissue distribution of enrofloxacin ( EF ) were investigated after single intramuscular (i.m.) dose of 10 mg/kg body weight (b.w.) in Pacific white shrimp at 22 to 25°C. EF and its metabolite ciprofloxacin ( CF ) were determined by high‐performance liquid chromatography. After i.m. administration, EF was absorbed quickly, and the peak of EF concentration ( C max ) reached at first time point in hemolymph. The volume of distribution V d(area) of EF was 3.84 L/kg, indicating that the distribution of EF was good. The area under the concentration–time curve ( AUC ) of EF was 90.1 and 274.2 μg hr/ml in muscle and hepatopancreas, respectively, which was higher than 75.8 μg hr/ml in hemolymph. The EF elimination was slow in muscle and hepatopancreas with the half‐life ( T 1/2β ) of 52.3 and 75.8 hr, respectively. CF , the mainly metabolite of EF , was detected in hemolymph, muscle and hepatopancreas. The C max was 0.030, 0.013 and 0.218 μg/ml, respectively. Based on a minimum inhibitory concentration ( MIC ) of 0.006–0.032 μg/ml for susceptible strains, EF i.m. injected at a dose 10 mg/kg could be efficacious against common pathogenic bacteria of Pacific white shrimp.