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Use of RNA ‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles
Author(s) -
Visser M.,
Weber K.,
Rincon G.,
Merritt D.
Publication year - 2017
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12400
Subject(s) - messenger rna , cytochrome p450 , kidney , duodenum , lung , microbiology and biotechnology , rna , biology , medicine , endocrinology , gene , biochemistry , metabolism
RNA sequencing ( RNA ‐seq) is a powerful tool for the evaluation and quantification of transcriptomes and expression patterns in animals, tissues, or pathological conditions. The purpose of this study was to determine the physiologic expression of cytochrome P450 ( CYP ) mRNA transcripts in whole blood, kidney, duodenum, liver, and lung in healthy, adult male ( n = 4) and female ( n = 4) beagles via RNA ‐seq. mRNA expression was above background (transcripts per million) for 45 canine CYP s, with liver, duodenum, and lung expressing a high number of xenobiotic metabolizing CYP s, while prominent endogenous metabolizing CYP expression was present in blood and kidney. The relative expression pattern of CYP 2A13 , 2B11 , 2C21 , 2D15 , 2E1 , 3A12, and 27A1 in liver, lung, and duodenum was verified through qPCR . This is the first global profiling of physiologic CYP mRNA expression in multiple canine tissues, providing a platform for further studies characterizing canine CYP s and changes in gene expression in disease states.