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Lung microdialysis study of florfenicol in pigs after single intramuscular administration
Author(s) -
Yang B.,
Gao J. D.,
Cao X. Y.,
Wang Q. Y.,
Sun G. Z.,
Yang J. J.
Publication year - 2017
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12387
Subject(s) - pharmacokinetics , florfenicol , microdialysis , elimination rate constant , chemistry , lung , high performance liquid chromatography , area under the curve , chromatography , pharmacology , volume of distribution , medicine , antibiotics , biochemistry , extracellular
For most bacterial lung infections, the concentration of unbound antimicrobial agent in lung interstitial fluid has been considered as the gold standard for estimating the antibacterial efficacy. In this study, the pharmacokinetics of florfenicol ( FF ) in porcine lung interstitial fluid was investigated after single intramuscular administration at two different doses (20 and 50 mg/kg). Twelve pigs underwent thoracotomy under general anesthesia. Then, the CMA /30 probe was implanted into the lung and perfused at 1 μL/min. The microdialysis ( MD ) samples were collected on a preset schedule and analyzed by high‐performance liquid chromatography ( HPLC ). Noncompartmental pharmacokinetic analysis was performed. FF exhibited rapid distribution and slow elimination in porcine lung interstitial fluid. The main pharmacokinetic parameters at 20 and 50 mg/kg were 4.88 ± 0.54 and 10.36 ± 2.52 μg/ mL for the maximum concentration (C max ), 3.25 ± 0.32 and 3.50 ± 0.27 h for the time to C max (T max ), 9.47 ± 6.84 and 7.75 ± 3.23 h for the half‐life (t 1/2 ), 0.10 ± 0.06 and 0.10 ± 0.04 1/h for the terminal elimination rate constant ( λ z ), 13.85 ± 7.97 and 11.42 ± 2.79 h for the mean residence time ( MRT ), 37.77 ± 8.13 and 71.15 ± 16.99 h·μg/ mL for the area under the curve from time 0 to 18.25 h ( AUC 0–18.25 ), and 51.18 ± 20.11 and 88.78 ± 27.58 h·μg/ mL for the area under the curve from time 0 to infinity ( AUC 0–∞ ), respectively.