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Two fluoroquinolones and their combinations with hyaluronan: comparison of effects on canine chondrocyte culture
Author(s) -
Siengdee P.,
Euppayo T.,
Buddhachat K.,
Chomdej S.,
Nganvongpanit K.
Publication year - 2016
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12305
Subject(s) - viability assay , chondrocyte , matrix metalloproteinase , apoptosis , downregulation and upregulation , hyaluronic acid , extracellular matrix , chemistry , tumor necrosis factor alpha , microbiology and biotechnology , pharmacology , immunology , medicine , biology , gene , biochemistry , in vitro , anatomy
Fluoroquinolones ( FQ s) are frequently used for septic arthritis. Increased antibacterial activity has been associated with mammalian cell cytotoxicity that may increase the risk of developing osteoarthritis. This study compared the direct effects of two different FQ s, enrofloxacin (Enro) and marbofloxacin (Mar), on normal primary canine chondrocytes and inflammatory‐stimulated chondrocytes, in addition to their administration in combination with hyaluronan ( HA ). Cell viability, cell apoptosis, s‐ GAG production, and expression patterns of inflammatory, extracellular matrix ( ECM ) component and protease genes were measured. Enro co‐culturing with HA could modify s‐ GAG synthesis compared with the negative control group. Co‐treatment with both FQ s and HA significantly decreased cell viability and induced more total apoptotic cell death compared with the negative control and pre‐ IL ‐1β‐stimulated group. Enro regulated IL ‐1β‐stimulated cells to overexpress IL ‐1β , TNF , and MMP 3 , whereas Mar induced upregulation of PTGS 2 and NFKB 1 and enhanced the expression of ECM component genes HAS 1 , COL 2A1, and ACAN as well as TIMP 1 and MMP 9 . Simultaneous use of HA with Enro can effectively reduce the expression of IL ‐1β, TNF , and MMP 3 in pre‐ IL ‐1β‐stimulated chondrocytes. These results suggest the beneficial effects of HA in reducing the adverse effects of Enro treatment at the transcriptional level.