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Pharmacokinetics of tildipirosin in pig tonsils
Author(s) -
Torres F.,
Santamaria R.,
Jimenez M.,
Menjón R.,
Ibanez A.,
Collell M.,
Azlor O.,
Fraile L.
Publication year - 2016
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12258
Subject(s) - tonsil , pharmacokinetics , saline , pentobarbital , chemistry , penetration (warfare) , body weight , plasma concentration , medicine , zoology , chromatography , nuclear medicine , pathology , biology , operations research , engineering
The penetration of antimicrobials in pig tonsils is hardly known. The objective of the study was to quantify the tildipirosin ( TD ) penetration in tonsils. Animals were randomly divided into six groups (control, T1, T2 (1), T2(5), T2(10), and T2(15)) of eight animals. T1 and T2 groups received a dose of 2 and 4 mg of TD /kg bw in one shot (Zuprevo ® MSD Animal Health), respectively, and the control group received 2 mL of saline solution. The animals were sacrificed by intravenous administration of pentobarbital sodium 24 h after finishing the treatment for the control, T1, and T2(1) groups, whereas animals of T2(5), T2(10), and T2(15) groups were sacrificed at 5, 10, and 15 days, post‐treatment, respectively. Tonsils and blood samples were taken at necropsy to obtain plasma, and the tildipirosin concentration was determined by high‐performance liquid chromatography with tandem mass spectrometry detection. The concentration in plasma was always significantly lower than in tonsil. Average TD tonsil concentrations increased significantly in a dose‐dependent manner, and the tonsil TD vs. plasma TD concentration ratio was approximately 75 for the doses of 2 and 4 mg of TD /kg bw at 24 h post‐treatment. Moreover, the maximum concentration of tildipirosin in tonsil was observed at 1 day postadministration, and this concentration decreased gradually from this day until 15 days postadministration for the dose of 4 mg of TD /kg bw. Finally, the ratio AUC tonsil / AUC plasma was 97.9, and the T 1/2 (h) was clearly higher in tonsil than in plasma.