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Pharmacokinetic study of enrofloxacin in Nile tilapia ( Oreochromis niloticus ) after a single oral administration in medicated feed
Author(s) -
Teles J. A.,
Castello Branco L. C.,
Del Bianchi M.,
Pilarski F.,
Reyes F. G. R.
Publication year - 2016
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12257
Subject(s) - oreochromis , nile tilapia , enrofloxacin , pharmacokinetics , oral administration , pharmacology , medicine , oral dose , veterinary medicine , biology , fish <actinopterygii> , fishery , microbiology and biotechnology , antibiotics , ciprofloxacin
The objective of this study was to evaluate the disposition kinetics of enrofloxacin ( ENR ) in the plasma and its distribution in the muscle tissue of Nile tilapia ( Oreochromis niloticus ) after a single oral dose of 10 mg/kg body weight via medicated feed. The fish were kept at a temperature between 28 and 30 °C. The collection period was between 30 min and 120 h after administration of the drug. The samples were analyzed by high‐performance liquid chromatography with a fluorescence detector ( HPLC ‐ FLD ). The ENR was slowly absorbed and eliminated from the plasma ( C max = 1.24 ± 0.37 μ g/mL; T max = 8 h; T 1/2 K e = 19.36 h). ENR was efficiently distributed in the muscle tissue and reached maximum values (2.17 ± 0.74 μ g/g) after 8 h. Its metabolite, ciprofloxacin ( CIP ), was detected and quantified in the plasma (0.004 ± 0.005 μ g/mL) and muscle (0.01 ± 0.011 μ g/g) for up to 48 h. After oral administration, the mean concentration of ENR in the plasma was well above the minimum inhibitory concentrations ( MIC 50 ) for most bacteria already isolated from fish except for Streptococcus spp. This way the dose used in this study allowed for concentrations in the blood to treat the diseases of tilapia.