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Depletion of melamine and cyanuric acid in kidney of catfish I ctalurus punctatus and trout O ncorhynchus mykiss
Author(s) -
Stine C. B.,
Nochetto C.,
Gieseker C. M.,
Evans E. R.,
Hasbrouck N. R.,
Mayer T. D.,
Girard L.,
Reimschuessel R.
Publication year - 2013
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12058
Subject(s) - cyanuric acid , melamine , ictalurus , catfish , rainbow trout , chemistry , residue (chemistry) , kidney , body weight , trout , biochemistry , food science , fish <actinopterygii> , zoology , fishery , biology , endocrinology , organic chemistry
A risk assessment conducted in 2007 identified significant knowledge gaps about tissue residues of melamine and related triazine analogs such as cyanuric acid in animals that had eaten contaminated food. The USFDA subsequently designed studies to determine residue levels in muscle, serum, and kidneys of catfish and trout given a single gavage dose of 20 mg/kg body weight ( BW ) of melamine, cyanuric acid, or 20 mg/kg BW of both compounds simultaneously. Renal triazines were determined by LC ‐ MS / MS at postdose days 1, 3, 7, 14, 28 (and day 42 for trout). When dosed individually, melamine and cyanuric acid kidney residues depleted much faster than those in fish given both compounds together. Combined dose residue depletion was punctuated by extreme outliers due to the formation of persistent renal melamine cyanurate crystals.