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Hepatic and intestinal CYP 3 A expression and activity in broilers
Author(s) -
Osselaere A.,
De Bock L.,
Eeckhaut V.,
De Backer P.,
Van Bocxlaer J.,
Boussery K.,
Croubels S.
Publication year - 2013
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12034
Subject(s) - jejunum , ileum , cyp3a , duodenum , small intestine , drug metabolism , broiler , microsome , medicine , biology , metabolism , chemistry , in vitro , biochemistry , zoology
Cytochrome P 450 is involved in drug metabolism. Subfamily CYP 3 A shows a degree of similarity across different animal species. However, little information is available about its expression and activity in broiler chickens. A RT ‐ PCR method was developed for the quantification of CYP 3 A 37 expression in the liver and small intestine of broilers. A higher expression in the jejunum was observed compared with that in the ileum. In the liver, a significantly lower expression compared with that in the jejunum was noticed. Thus, the role of the small bowel in drug metabolism cannot be neglected in broilers. CYP 3 A activity was studied in vitro using midazolam as a substrate. Two protocols for the preparation of intestinal microsomes were compared. Mincing of the tissues before ultracentrifugation seemed to be more appropriate than a protocol based on ethylenediaminetetra‐acetic acid separation. CYP 3 A activity revealed to be the highest in the duodenum with a decreasing trend towards the ileum. Activity in liver was comparable to duodenal activity.