Open AccessVariability of Symmetric Dimethylarginine in Apparently Healthy Dogs
Author(s) -
Kopke M.A.,
Burchell R.K.,
Ruaux C.G.,
Burton S.E.,
LopezVillalobos N.,
Gal A.
Publication year - 2018
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.15050
Subject(s) - medicine , creatinine , population , observational study , kidney disease , confidence interval , renal function , environmental health
Background Symmetric dimethylarginine (SDMA) is a screening tool for early kidney dysfunction and monitoring treatment in cases of chronic kidney disease (CKD). There are no current studies describing the suitability of this test for use with published population‐based reference intervals. Hypothesis/Objectives To determine the components of biological variability, the index of individuality (IOI), the critical difference between sequential measurements ( C D ) and the number of measurements required to assess the homeostatic set point (HSP), for both SDMA and serum creatinine (sCr), in apparently healthy dogs. Animals Twenty apparently healthy adult dogs owned by clients or staff at a veterinary teaching hospital. Methods Prospective, observational study. Blood was collected from each dog on 9 occasions, and SDMA and sCr were measured in duplicate using commercially available assays. Results SDMA and sCr had intermediate and low IOI values of 0.87 and 0.28, respectively. The C D of SDMA and sCr, was 1.34 µg/dL and 0.89 µmol/L, respectively. The sample numbers required for estimation of an individual's HSP (with 90 and 95% CI) for SDMA and sCr were 8 and 45, and 2 and 12 sequential measurements, respectively. Conclusions and Clinical Importance Based on our findings, in comparison to sCr, SDMA is better suited for use with population‐based reference intervals. False‐negative test results could occur when comparing a single test result from an individual to such intervals. Ideally C D should be used with sequential measurements.