Open AccessUrinary F 2 ‐Isoprostanes in Cats with International Renal Interest Society Stage 1–4 Chronic Kidney Disease
Author(s) -
Whitehouse W.,
Quimby J.,
Wan S.,
Monaghan K.,
Robbins R.,
Trepanier L.A.
Publication year - 2017
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.14634
Subject(s) - cats , medicine , kidney disease , creatinine , urinary system , proteinuria , renal function , urine specific gravity , endocrinology , urine , urology , biomarker , oxidative stress , kidney , gastroenterology , biology , biochemistry
Background F 2 ‐isoprostanes, a biomarker of oxidant injury, increase with advancing chronic kidney disease ( CKD ) in humans. In cats, the relationship between CKD and oxidative stress is poorly understood. Objectives To determine whether cats with advancing CKD have increasing urinary F 2 ‐isoprostanes. Animals Control cats without evidence of CKD (≥6 years old; n = 11), and cats with IRIS stage 1 (n = 8), 2 (n = 38), 3 (n = 21), and 4 (n = 10) CKD . Methods This was a prospective observational study. Urinary F 2 ‐isoprostanes (specifically free 15‐F 2t ‐isoprostanes) normalized to urine creatinine (IsoPs) were compared among groups and tested for correlations with blood pressure, proteinuria, serum creatinine concentration, and urine specific gravity. The IsoPs also were compared between cats with and without hypertension or proteinuria, and in cats fed predominantly standard versus renal diets. Results Urinary IsoPs were increased, but not significantly, in cats with stage 1 CKD (median 263 pg/mg creatinine; range, 211–380) compared to controls (182 pg/mg; range, 80–348) and decreased significantly from stage 1 through advancing CKD (stage 2, 144 pg/mg; range, 49–608; stage 3, 102 pg/mg; range, 25–158; stage 4, 67 pg/mg; range, 26–117; P < .01). Urinary IsoPs were inversely correlated with serum creatinine ( r = −0.66, P < .0001). Conclusion and Clinical Importance Urinary IsoPs are significantly higher in early CKD (stage 1) compared to cats with more advanced CKD . Additional studies are warranted to characterize oxidative stress in cats with stage 1 CKD and determine whether early antioxidant treatments have a protective effect on CKD progression.