English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Background  Canine adenovirus 2, parainfluenza, and   B ordetella bronchiseptica  cause respiratory disease in dogs, and each has a modified live intranasal vaccine available. Molecular diagnostic assays to amplify specific nucleic acids are available for each of these agents. If positive molecular diagnostic assay results are common after vaccination, the positive predictive value of the diagnostic assays for disease would be decreased.    Objective  To determine the impact of administration of commercially available modified live topical adenovirus 2,   B . bronchiseptica , and parainfluenza vaccine has on the results of a commercially available  PCR  panel.    Animals  Eight puppies from a research breeding facility negative for these pathogens.    Methods  Blinded prospective pilot study. Puppies were vaccinated with a single dose of modified live topical adenovirus 2,   B . bronchiseptica , and parainfluenza and parenteral dose of adenovirus 2, canine distemper virus, and parvovirus. Nasal and pharyngeal swabs were collected on multiple days and submitted for  PCR  assay.    Results  Nucleic acids of all 3 organisms contained in the topical vaccine were detected from both samples multiple times through 28 days after vaccination with higher numbers of positive samples detected between days 3 and 10 after vaccination.    Conclusions and Clinical Importance  Vaccine status should be considered when interpreting respiratory agent  PCR  results if modified live vaccines have been used. Development of quantitative  PCR  and wild‐type sequencing are necessary to improve positive predictive value of these assays by distinguishing vaccinate from natural infection.

journal of veterinary internal medicine

Adenovirus 2,   B ordetella bronchiseptica , and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines