Open AccessISO ‐Based Assessment of Accuracy and Precision of Glucose Meters in Dogs
Author(s) -
BritoCasillas Y.,
Figueirinhas P.,
Wiebe J.C.,
LópezRíos L.,
PérezBarreto D.,
Melián C.,
Wägner A.M.
Publication year - 2014
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12397
Subject(s) - hematocrit , medicine , accuracy and precision , plasma glucose , coefficient of variation , limits of agreement , zoology , reference values , diagnostic accuracy , nuclear medicine , statistics , mathematics , biology , insulin
Background Portable blood glucose meters ( PBGM s) allow easy glucose measurements. As animal‐specific PBGM s are not available everywhere, those for humans are widely used. Objectives To assess the accuracy and precision of 9 PBGM s in canine whole blood ( WB ) and plasma, based on the ISO 15197:2013. Animals Fifty‐nine client‐owned dogs attending the Veterinary Teaching Hospital. Methods Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29–579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase‐based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences. Results Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM ( r = −0.243 − [−0.371]; P < .021). Conclusions and Clinical Importance This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species.