Open AccessCanine T‐Zone Lymphoma: Unique Immunophenotypic Features, Outcome, and Population Characteristics
Author(s) -
Seelig D.M.,
Avery P.,
Webb T.,
Yoshimoto J.,
Bromberek J.,
Ehrhart E.J.,
Avery A.C.
Publication year - 2014
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12343
Subject(s) - immunophenotyping , medicine , lymphocytosis , lymphoma , canine lymphoma , pathology , population , lymph node , flow cytometry , immunology , environmental health
Background Canine T‐cell lymphoma ( TCL ) is clinically and histologically heterogeneous with some forms, such as T‐zone lymphoma ( TZL ), having an indolent course. Immunophenotyping is an important tool in the classification of TCL in people, and can be equally useful in dogs. Hypothesis/Objectives We hypothesized that loss of expression of the CD 45 antigen is a specific diagnostic feature of TZL . Animals Twenty dogs with concurrent histology and immunophenotyping by flow cytometry were studied in depth. An additional 494 dogs diagnosed by immunophenotyping were used to characterize the population of dogs with this disease. Methods Lymph node biopsies from 35 dogs with TCL were classified by 2 pathologists using WHO criteria. Twenty lymph nodes were from dogs with CD 45− TCL and 15 were from CD 45+ TCL . The pathologists were blinded to the flow cytometry findings. Outcome information was sought for the 20 dogs with CD 45− lymphoma, and population characteristics of the additional 494 dogs were described. Results All 20 CD 45− cases were classified as TZL . The 15 CD 45+ cases were classified as aggressive TCL and are described in an accompanying paper. TZL cases had a median survival of 637 days. Examination of 494 additional dogs diagnosed with TZL by immunophenotyping demonstrated that 40% of cases are in Golden Retrievers, are diagnosed at a median age of 10 years, and the majority have lymphadenopathy and lymphocytosis. Conclusions TZL has unique immunophenotypic features that can be used for diagnosis.