Open AccessDEA 1 Expression on Dog Erythrocytes Analyzed by Immunochromatographic and Flow Cytometric Techniques
Author(s) -
Acierno M.M.,
Raj K.,
Giger U.
Publication year - 2014
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12321
Subject(s) - polyclonal antibodies , flow cytometry , antigen , antibody , typing , monoclonal antibody , microbiology and biotechnology , medicine , blood bank , immunology , biology , genetics , medical emergency
Background The Dog erythrocyte antigen ( DEA ) 1 blood group system was thought to contain types DEA 1.1 and 1.2 (and possibly 1.3 [A 3 ]). However, DEA 1.2+ dogs are very rare and newer typing methods reveal varying degrees of DEA 1 positivity. Objectives To assess if variation in DEA 1 positivity is because of quantitative differences in surface antigen expression. To determine expression patterns in dogs over time and effects of blood storage (4°C). To evaluate DEA 1.2+ samples by DEA 1 typing methods. Animals Anticoagulated blood samples from 66 dogs in a research colony and from a hospital, and 9 previously typed DEA 1.2+ dogs from an animal blood bank. Methods Research study: Samples were analyzed by flow cytometry and immunochromatographic strip using a monoclonal anti‐ DEA 1 antibody. Results Twenty dogs were DEA 1−, whereas 46 dogs were weakly to strongly DEA 1+. Antigen quantification revealed excellent correlation between strip and flow cytometry ( r = 0.929). Both methods reclassified DEA 1.2+ samples as weakly to moderately DEA 1+, but they were not retyped with the polyclonal anti‐ DEA 1.1/1.X antibodies. Dogs and blood samples retained their relative DEA 1 antigen densities over time. Conclusions and Clinical Importance The blood group system DEA 1 is a continuum from negative to strongly positive antigen expression. Previously typed DEA 1.2+ appears to be DEA 1+. These findings further the understanding of the DEA 1 system and suggest that all alleles within the DEA 1 system have a similarly based epitope recognized by the monoclonal antibody.