Open AccessEffect of Trilostane and Mitotane on Aldosterone Secretory Reserve in Dogs with Pituitary‐Dependent Hyperadrenocorticism
Author(s) -
Reid L.E.,
Behrend E.N.,
Martin L.G.,
Kemppainen R.J.,
Ward C.R.,
Lurye J.C.,
Donovan T.C.,
Lee H.P.
Publication year - 2014
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12276
Subject(s) - aldosterone , medicine , endocrinology , mitotane , stimulation , chemotherapy
Background Maximal aldosterone secretion in healthy dogs occurs 30 minutes postadrenocorticotropin ( ACTH ; 5 μg/kg IV) stimulation. The effect of trilostane and mitotane on aldosterone at that time is unknown. Objectives To assess the effect of trilostane and mitotane in dogs with pituitary‐dependent hyperadrenocorticism on aldosterone secretory reserve. To determine if aldosterone concentration correlates with electrolyte concentrations. Animals Serum collected from 79 client‐owned dogs and 33 stored samples. Methods Client‐owned dogs had ACTH stimulation tests with cortisol concentrations measured at 0 and 60 minutes and aldosterone concentrations measured at 0, 30, and 60 minutes. Stored samples had aldosterone concentrations measured at 0 and 60 minutes. Ten historical clinically healthy controls were included. All had basal sodium and potassium concentrations measured. Results The aldosterone concentrations in the mitotane‐ and trilostane‐treated dogs at 30 and 60 minutes post‐ ACTH were significantly lower than in clinically healthy dogs; no significant difference was detected in aldosterone concentration between 30 and 60 minutes in treated dogs. However, a significantly higher percentage of dogs had decreased aldosterone secretory reserve detected at 30 minutes than at 60 minutes. At 30 minutes, decreased secretory reserve was detected in 49% and 78% of trilostane‐ and mitotane‐treated dogs, respectively. No correlation was detected between aldosterone and serum electrolyte concentrations. Conclusions and Clinical Importance Decreased aldosterone secretory reserve is common in trilostane‐ and mitotane‐treated dogs; it cannot be predicted by measurement of serum electrolyte concentrations. Aldosterone concentration at 30 minutes post‐ ACTH stimulation identifies more dogs with decreased aldosterone secretory reserve than conventional testing at 60 minutes.