Open AccessAnalysis of Seroreactivity against Cell Culture–Derived B artonella spp. Antigens in Dogs
Author(s) -
Hegarty B.C.,
Bradley J.M.,
Lappin M.R.,
Balakrishnan N.,
Mascarelli P.E.,
Breitschwerdt E.B.
Publication year - 2013
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12263
Subject(s) - bartonella , antigen , serology , genotype , serotype , virology , antibody , microbiology and biotechnology , bartonella henselae , bacteremia , medicine , biology , immunology , gene , antibiotics , biochemistry
Background Little is known about the specificity of Bartonella spp. immunofluorescent antibody ( IFA ) assays in dogs. Bacteremia in sick dogs most often has been associated with Bartonella henselae ( Bh ) , Bartonella vinsonii subspecies berkhoffii ( Bvb ), and Bartonella koehlerae ( Bk ). Clarification of the diagnostic utility of IFA serology when testing against these organisms is needed. Objective To evaluate the specificity of Bartonella IFA assays utilizing 6 cell culture–grown antigen preparations. Animals Archived sera from SPF dogs (n = 29) and from dogs experimentally infected with Bvb (n = 10) and Bh (n = 3). Methods Antibodies (Abs) to Bvb genotypes I, II, and III, Bh serotype I, strains H‐1 and SA 2, and to Bk were determined by IFA testing. Results Serum from naïve SPF dogs shown to be negative for Bartonella bacteremia did not react with any of the 6 Bartonella antigens by IFA testing. Dogs experimentally infected with Bvb genotype I developed Abs against homologous antigens, with no cross‐reactivity to heterologous Bvb genotypes, Bh H‐1, SA 2 strains, or to Bk . Dogs experimentally infected with Bh serotype I developed Abs against Bh H‐1, but not to Bh SA 2 strain with no cross‐reactive Abs to Bvb genotypes I– III or to Bk . Conclusions and Clinical Importance Bartonella spp. Ab responses during acute experimental infections are species and type specific.