Open AccessEvaluation of 4 Point‐of‐Care Units for the Determination of Blood l ‐Lactate Concentration in Cattle
Author(s) -
Karapinar T.,
Kaynar O.,
Hayirli A.,
Kom M.
Publication year - 2013
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.12204
Subject(s) - medicine , blood lactate , point of care , jugular vein , zoology , anaerobic exercise , chromatography , chemistry , pathology , biology , physiology , heart rate , blood pressure
Background Despite being used commonly in bovine medicine, information on reliability of point‐of‐care ( POC ) lactate meters is limited. Objective To determine the validity of 4 commercially available POC lactate meters in cattle. Animals Cattle with various diseases (n = 120). Methods Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma l ‐lactate concentration was measured by the enzymatic‐colorimetric method ( ELISA ). Data were subjected to Friedman's test for comparison, Passing‐Bablok regression and Bland‐Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity ( Se ) and specificity ( Sp ). Results The POC lactate meters were highly correlated with ELISA ( r = 0.98–0.99) despite disagreements among units. When regressed on ELISA , blood l ‐ lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i‐ STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7–98.6% at a plasma l ‐lactate cut‐off concentration of 4 mmol/L. i‐ STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit ( y = −0.13 + 1.04 X ) yielding the lowest bias (−6.6%) as well as the highest Se (100%) and Sp (98.6%). Conclusions and Clinical Importance Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i‐ STAT /Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i‐ STAT provided the most accurate and precise results.