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HBV coinfection with HCV alters circulating Tfh cell distribution and impairs HCV neutralizing antibody responses
Author(s) -
Wen Bo,
Zhang Jian,
Liu Wenpei,
Tang Ping,
Xie Ting,
Huang Liyan,
Hu Yabin,
Jin Kun,
Zhang Ping,
Liu Ziyan,
Niu Ling,
Qu Xiaowang
Publication year - 2019
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.13106
Subject(s) - coinfection , neutralizing antibody , antibody , virology , hepatitis c virus , hepatitis b virus , medicine , immunology , hepatitis b , virus
Hepatitis C virus ( HCV ) and hepatitis B virus ( HBV ) coinfection reciprocally influences viral replication and host defence responses. This study aimed to investigate the impact of HBV coinfection on circulating T follicular helper cell ( cT fh) distribution and the HCV neutralizing antibody ( nA b) response. HCV neutralizing antibody responses were measured in individuals with HCV monoinfection (n = 83) and HBV / HCV coinfection (n = 78) using the HCV pseudoparticle neutralization assay. The frequencies of cT fh cells and their subsets in HCV monoinfection (n = 34) and HBV / HCV coinfection (n = 30) were analysed by flow cytometry. The correlations of clinical parameters, cT fh cells and neutralizing antibody responses were analysed. Compared with HCV monoinfection, the HBV coinfection group showed significantly lower HCV neutralizing antibody responses ( P < 0.001) and a decreased frequency of circulating Th1‐like Tfh cells (Tfh1) ( P = 0.004). In HCV monoinfection, the frequency of the Tfh1 subset was positively correlated with HCV neutralizing antibody responses ( R = 0.378, P = 0.03), but this correlation was lost under HBV / HCV coinfection ( R = 0.115, P = 0.551). In contrast, the frequency of circulating Th2‐like Tfh cells (Tfh2) was negatively correlated with the HCV neutralizing antibody responses ( R = 0.404, P = 0.003). Further analysis showed that HBV coinfection enhanced the Tfh2 subset composition within cT fh cells ( P < 0.001), which was associated with serum HB sAg in HBV / HCV coinfection ( R = 0.521, P = 0.003). As expected, HB sAg also exhibited an inverse association with HCV neutralizing antibody responses in HBV / HCV coinfection ( R = 0.59, P < 0.001). In contrast to HCV monoinfection, HBV / HCV coinfection leads to altered cT fh cell distribution and impaired HCV neutralizing antibody responses, which are associated with HB sAg. These findings will be helpful for better understanding the immunopathogenesis of HBV / HCV coinfection.