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Lnc RNA expression profiles in HBV ‐transformed human hepatocellular carcinoma cells treated with a novel inhibitor of human La protein
Author(s) -
Pan J.,
Tong S.,
Tang J.
Publication year - 2018
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12821
Subject(s) - biology , wnt signaling pathway , microbiology and biotechnology , rna , microarray analysis techniques , messenger rna , downregulation and upregulation , gene expression , rna interference , gene silencing , signal transduction , gene , genetics
Summary We previously identified a novel inhibitor of La protein, H11, which inhibited hepatitis B virus ( HBV ) replication by inhibiting the interaction between La protein and HBV RNA . However, the other cellular factors involved in this process remain unclear. To investigate the mechanism of H11‐mediated inhibition of HBV infection, a lnc RNA microarray analysis was performed using H11‐treated and untreated stable HBV ‐expressing human hepatoblastoma HepG2.2.15 cells. The profiles of differentially expressed lnc RNA s and mRNA s were generated and analysed using Gene Ontology ( GO ) and pathway analyses. The microarray data showed that 61 lnc RNA s were upregulated, 74 lnc RNA s were downregulated, 43 mRNA s were upregulated, and 44 mRNA s were downregulated in H11 treatment group when compared with the control group, and these results were consistent with qRT ‐ PCR expression data. Bioinformatic analysis indicated that the differentially expressed lnc RNA s were involved in RNA ‐mediated post‐transcriptional gene silencing, regulation of viral genome replication and Jak‐ STAT signalling and apoptosis pathways. GO analysis showed that differentially expressed mRNA s were enriched in negative regulation of the Wnt signalling pathway and negative regulation of growth. Pathways analysis indicated that the differentially expressed mRNA s were involved in regulation of nuclear β‐catenin signalling and target gene transcription, as direct p53 effectors, and in the peroxisome proliferator‐activated receptors signalling and peroxisome pathways. Microarray data and qRT ‐ PCR results indicated that H11 mediates inhibition of HBV replication by regulating the Wnt, β‐catenin and PPAR signalling pathways.