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Alpha‐enolase regulates hepatitis B virus replication through suppression of the interferon signalling pathway
Author(s) -
XiangChun D.,
Xiaoqing Y.,
TingTing Y.,
ZhenHui L.,
XiaoYan L.,
Xia L.,
YanChao H.,
YiXuan Y.,
LiNa M.
Publication year - 2018
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12813
Subject(s) - hepatitis b virus , virology , interferon , downregulation and upregulation , biology , gene silencing , transfection , viral replication , hepatitis b virus pre beta , microbiology and biotechnology , virus , cell culture , gene , hepatitis b virus dna polymerase , biochemistry , genetics
Summary Persistent chronic infection with hepatitis B virus ( HBV ) is a major risk factor for the development of HBV ‐related diseases. The molecular mechanisms that underlie HBV infection and associated carcinogenesis are not fully understood. The aim of this study was to explore the role of ENO 1 in HBV replication processes. Here, we examined ENO 1 expression levels in HBV ‐infected and non‐ HBV ‐infected liver tissues and cells by Western blot analysis, real‐time PCR and immunohistochemistry. In addition, HB sAg and HB eAg in the media of transfected HepG2.2.15 cells were detected using an electrochemical luminescence analyser within 48 hours after ENO 1‐specific si RNA transfection. The expression levels of HBV DNA , type I interferon and 5 downstream IFN ‐stimulated genes in HepG2.2.15 cells were examined using real‐time PCR . We found ENO 1 expression was upregulated in the HBV ‐infected liver tissues and cells. Silencing of ENO 1 resulted in a significant reduction in HBV replication, and this si RNA ‐mediated reaction also caused the upregulation of expression of type I interferon and downstream IFN ‐stimulated genes. Therefore, we come to the conclusion ENO 1 is involved in HBV replication. It is therefore likely that HBV replication is enhanced following suppression of the IFN signalling pathway. However, the mechanisms that underpin ENO 1‐mediated modulation of the IFN signalling pathway remain to be elucidated.