Premium
Associations of human leucocyte antigen class II ‐ DQB 1 alleles with hepatitis C virus infection in Egyptian population: a multicentre family‐based study
Author(s) -
ElBendary M.,
Neamatallah M.,
Esmat G.,
Kamel E.,
Elalfy H.,
Besheer T.,
Eldeib D.,
Eladl A.H.,
ElSetouhy M.,
ElGilany A.H.,
ElWaseef A.
Publication year - 2016
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12573
Subject(s) - hepatitis c virus , clearance , allele , genotype , human leukocyte antigen , immunology , medicine , antibody , virus , population , hepatitis c , virology , antigen , biology , gene , genetics , environmental health , urology
Summary Hepatitis C infection is a global pandemic. HLA ‐ DQB 1 alleles are believed to have an effective role in immune response against HCV including susceptibility to or protection from this infection. The aim of this study was to investigate the contribution of HLA ‐ DQB 1 alleles in the outcome of HCV genotype‐4 infection through a family‐based association study. Egyptian families with HCV (324) were recruited for this study (324 index positive for RNA ‐ HCV , 225 positive relatives representing chronic hepatitis C cases and 582 family members negative for HCV ‐ RNA [control], 63 of whom spontaneously cleared the virus. All subjects were genotyped for HLA ‐ DQB 1 alleles by sequence‐specific primers ( SSP ‐ PCR ) and sequence‐based typing ( SBT ) methods. The frequency of DQB 1*02:01:01 carriage was significantly higher in infected patients when compared to controls and those who spontaneously cleared virus ( OR =5.47, P <.0001 and OR = 6.5234, P <.0001, respectively), and the carriage of the DQB 1*03:01:01:01 allele was significantly higher in those who cleared and controls when compared to the infected patients ( OR =0.2889, P <.0001 and OR =0.3016, P <.0001, respectively). On the other hand, the frequency of DQB 1*06:01:01 and QB 1*05:01:01:01 alleles was not associated with infection (comparison of infected and cleared patients showed OR of 2.1598 [ P <.01]), but it becomes nonsignificant after adjustments with the Bonferroni formula (P C >0.05) and OR = 1.3523, P >.05, respectively. This study shows that clearance of HCV is associated with DQB 1*03:01:01:01 allele and chronicity of HCV infection associated with the risk allele: DQB 1*02:01:01.