Premium
The expression of miR‐125b‐5p is increased in the serum of patients with chronic hepatitis B infection and inhibits the detection of hepatitis B virus surface antigen
Author(s) -
Ninomiya M.,
Kondo Y.,
Kimura O.,
Funayama R.,
Nagashima T.,
Kogure T.,
Morosawa T.,
Tanaka Y.,
Nakayama K.,
Shimosegawa T.
Publication year - 2016
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12522
Subject(s) - rna , virology , microrna , hepatitis b virus , messenger rna , microbiology and biotechnology , downregulation and upregulation , oligonucleotide , biology , virus , gene , genetics
Summary Micro RNA s were first discovered as small endogenous RNA molecules and some viruses have been reported to interact with host mi RNA s. By investigating mi RNA expression in serum derived from HBV ‐infected patients, we have clarified the relationship between mi RNA expression and chronic HBV infection. Additionally, we demonstrate the use of mi RNA s as both novel biomarkers and new therapies against HBV . We included the sera of 20 patients with chronic HBV infection, sera of 20 patients with HCV infection and sera of 10 healthy controls in this study. The mi RNA libraries were sequenced using a 32‐mer single end sequence. The validation study of circulating mi RNA in serum was conducted by qRT ‐ PCR . The HBV genomic regions of genotype B and genotype C that were speculated to be targeted by mi RNA were constructed using complementary oligonucleotides in the vectors. Reporter assays were performed 48 h after transfection. The expression levels of 21 mi RNA s were found to be differentially expressed in the three groups. 10 mi RNA s (hsa‐miR‐100‐5p, miR‐125b‐5p, miR‐193b‐3p, miR‐194‐3p, miR‐30a‐3p, miR‐30c‐2‐3p, miR‐3591‐5p, miR‐4709‐3p, miR‐574‐3p and miR‐99a‐5p) were found to be upregulated in CH ‐B by deep sequence analysis. The computer analysis showed that two regions of HB sAg are potential targets of miR‐125b‐5p and miR‐30c‐2‐3p and that these mi RNA s may downregulate the expression of HBV ‐S. The HBV genotype C segment speculated to be targeted by hsa‐miR‐125b‐5p significantly decreased the expression of the reporter. This study indicated that expression of miR‐125b‐5p was related to the etiology of chronic hepatitis B infection and regulated the expression of HB sAg.