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Serum‐derived hepatitis C virus 1a infection of human astrocyte cell line SVG
Author(s) -
Rajalakshmy A. R.,
Malathi J.,
Madhavan H. N.
Publication year - 2016
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12480
Subject(s) - virology , astrocyte , hepatitis c virus , viral replication , cell culture , virus , biology , cell , immunology , medicine , central nervous system , genetics , neuroscience
Summary Neuroinvasion of hepatitis C virus ( HCV ) is evidenced by recent clinical studies. In this study, serum‐derived HCV infection of astrocytes was analysed. Astrocytes were infected with HCV ‐positive serum, and viral replication was assessed on different days postinfection. RT ‐ PCR was positive for HCV ‐negative strand on 5th and 7th day postinfection in the HCV ‐positive serum‐infected astrocytes. Real‐time RNA count in the cell culture supernatant was steadily increasing from day 3 to day 7. To reconfirm the viral replication, astrocytes were treated with an antiviral before the serum infection, and the antiviral treatment significantly reduced the viral RNA count. Further, the virus‐infected cells stained positive for the presence of viral core protein. Electron microscopy revealed the presence of HCV ‐like particles in the astrocyte cell culture supernatant. In conclusion, serum‐derived HCV replicates in human astrocyte cell line SVG .