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Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in V ietnamese HB sAg‐positive patients
Author(s) -
Sy B. T.,
Nguyen H. M.,
Toan N. L.,
Song L. H.,
Tong H. V.,
Wolboldt C.,
Binh V. Q.,
Kremsner P. G.,
Velavan T. P.,
Bock C.T.
Publication year - 2015
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/jvh.12228
Subject(s) - genotype , hepatitis d virus , virology , biology , hepatitis d , superinfection , hepatitis b virus , virus , genetics , hbsag , gene
Summary Hepatitis D virus ( HDV ) infection is acquired as a co‐ /superinfection of H epatitis B virus ( HBV ) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV . With a recorded prevalence of 10–20% HBV infection in V ietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty‐one HDV isolates in V ietnamese HB sAg‐positive patients. HDV subgenomic and full‐length genome sequences were obtained using newly established HDV ‐specific RT ‐ PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full‐length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated V ietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes ( HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDA g coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a V ietnamese HB sAg‐positive patient. Extended investigation on the distribution and prevalence of HDV , HDV mixed genotypes and recombinant HDV genotypes in a larger V ietnamese population offers vital insights into understanding of the micro‐epidemiology of HDV and subsequent pathophysiology in chronic HBV ‐ / HDV ‐related liver diseases.