Open AccessDengue virus detection in Aedes aegypti larvae from southeastern Brazil
Author(s) -
Cecílio Samyra Giarola,
Júnior Willer Ferreira Silva,
Tótola Antônio Helvécio,
de Brito Magalhães Cíntia Lopes,
Ferreira Jaqueline Maria Siqueira,
de Magalhães José Carlos
Publication year - 2015
Publication title -
journal of vector ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.688
H-Index - 51
eISSN - 1948-7134
pISSN - 1081-1710
DOI - 10.1111/jvec.12134
Subject(s) - aedes aegypti , biology , dengue fever , dengue virus , aedes albopictus , larva , aedes , veterinary medicine , virology , transmission (telecommunications) , ecology , medicine , electrical engineering , engineering
The transmission of dengue, the most important arthropod‐borne viral disease in Brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its Aedes vectors. In the present study, we mapped dengue vectors in Ouro Preto and Ouro Branco, Minas Gerais, by installing ovitraps in 32 public schools. The traps were examined monthly between September, 2011 through July, 2012 and November, 2012 to April, 2013. The larvae were reared until the fourth stadium and identified according to species. The presence of dengue virus was detected by real time PCR and agarose gel electrophoresis. A total of 1,945 eggs was collected during the 17 months of the study. The Ovitrap Positivity Index (OPI) ranged from 0 to 28.13% and the Eggs Density Index (EDI) ranged from 0 to 59.9. The predominant species was Aedes aegypti , with 84.9% of the hatched larvae. Although the collection was low when compared to other ovitraps studies, vertical transmission could be detected. Of the 54 pools, dengue virus was detected in four Ae. aegypti pools .