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Comparison of methods for measuring shear force and sarcomere length and their relationship with sensorial tenderness of longissimus muscle in beef
Author(s) -
Battaglia Christian,
Vilella Gustavo F.,
Bernardo Ana P. S.,
Gomes Carolina L.,
Biase Adriele G.,
Albertini Tiago Z.,
Pflanzer Sergio B.
Publication year - 2020
Publication title -
journal of texture studies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.593
H-Index - 54
eISSN - 1745-4603
pISSN - 0022-4901
DOI - 10.1111/jtxs.12473
Subject(s) - tenderness , sarcomere , myofibril , repeatability , longissimus muscle , mathematics , zoology , chemistry , food science , medicine , biology , statistics , biochemistry , myocyte
Slice shear force (SSF) and laser diffraction, considered faster methodologies, for measuring beef instrumental tenderness and sarcomere length, were compared with reference methodologies Warner–Bratzler shear force (WBSF) and phase contrast microscopy. Striploin samples ( n = 74) were analyzed for pH, sarcomere length, instrumental tenderness, myofibrillar fragmentation index, and sensorial tenderness. Pearson's correlation measured the association of meat evaluation methods with residual analysis of the multivariate analysis of variance model. The n‐dimensional profile to evaluated methods was presented by biplot to identify the behavior of the correlation between the methods (variables). There was moderate correlation between SSF and WBSF ( r = .63; p < .01) and both presented moderate correlation with sensorial tenderness ( r = − .62 and −.55, to SSF and WBSF, respectively; p < .01). However, WBSF was more efficient to classify samples as tender (68%) than SSF (47%), comparing with sensorial tenderness (80%). There was a moderate correlation for laser and microscopy for sarcomere length ( r = .57; p < .01). Sarcomeres were shorter when measured by laser than microscopy. Either with low correlation coefficients, sarcomere measured by laser ( r = .29; p < .05) presented higher correlation with sensorial tenderness than with microscopy ( r = .22; p < .10). Results highlighted that SSF was faster and easier to run, while WBSF was more appropriate to classify samples by sensorial tenderness grades. Laser diffraction is more suitable to explain effects on tenderness; however, microscopy revealed results of sarcomere length that were more realistic, once laser can underestimate sarcomeres.