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Persistent endotheliopathy in the pathogenesis of long COVID syndrome
Author(s) -
Fogarty Helen,
Townsend Liam,
Morrin Hannah,
Ahmad Azaz,
Comerford Claire,
Karampini Ellie,
Englert Hanna,
Byrne Mary,
Bergin Colm,
O’Sullivan Jamie M.,
MartinLoeches Ignacio,
Nadarajan Parthiban,
Bannan Ciaran,
Mallon Patrick W.,
Curley Gerard F.,
Preston Roger J. S.,
Rehill Aisling M.,
McGonagle Dennis,
Ni Cheallaigh Cliona,
Baker Ross I.,
Renné Thomas,
Ward Soracha E.,
O’Donnell James S.
Publication year - 2021
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/jth.15490
Subject(s) - irish , pharmacy , medicine , family medicine , library science , philosophy , linguistics , computer science
Background Persistent symptoms including breathlessness, fatigue, and decreased exercise tolerance have been reported in patients after acute SARS‐CoV‐2 infection. The biological mechanisms underlying this “long COVID” syndrome remain unknown. However, autopsy studies have highlighted the key roles played by pulmonary endotheliopathy and microvascular immunothrombosis in acute COVID‐19. Objectives To assess whether endothelial cell activation may be sustained in convalescent COVID‐19 patients and contribute to long COVID pathogenesis. Patients and Methods Fifty patients were reviewed at a median of 68 days following SARS‐CoV‐2 infection. In addition to clinical workup, acute phase markers, endothelial cell (EC) activation and NETosis parameters and thrombin generation were assessed. Results Thrombin generation assays revealed significantly shorter lag times ( p < .0001, 95% CI −2.57 to −1.02 min), increased endogenous thrombin potential ( p = .04, 95% CI 15–416 nM/min), and peak thrombin ( p < .0001, 95% CI 39–93 nM) in convalescent COVID‐19 patients. These prothrombotic changes were independent of ongoing acute phase response or active NETosis. Importantly, EC biomarkers including von Willebrand factor antigen (VWF:Ag), VWF propeptide (VWFpp), and factor VIII were significantly elevated in convalescent COVID‐19 compared with controls ( p = .004, 95% CI 0.09–0.57 IU/ml; p = .009, 95% CI 0.06–0.5 IU/ml; p = .04, 95% CI 0.03–0.44 IU/ml, respectively). In addition, plasma soluble thrombomodulin levels were significantly elevated in convalescent COVID‐19 ( p = .02, 95% CI 0.01–2.7 ng/ml). Sustained endotheliopathy was more frequent in older, comorbid patients, and those requiring hospitalization. Finally, both plasma VWF:Ag and VWFpp levels correlated inversely with 6‐min walk tests. Conclusions Collectively, our findings demonstrate that sustained endotheliopathy is common in convalescent COVID‐19 and raise the intriguing possibility that this may contribute to long COVID pathogenesis.