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Stabilin‐2 deficiency increases thrombotic burden and alters the composition of venous thrombi in a mouse model
Author(s) -
Michels Alison,
Swystun Laura L.,
Dwyer Courtney N.,
Rawley Orla,
Nesbitt Kate,
Notley Colleen,
Lillicrap David
Publication year - 2021
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/jth.15429
Subject(s) - thrombus , von willebrand factor , medicine , thrombosis , pathogenesis , venous thrombosis , scavenger receptor , inferior vena cava , endocrinology , immunology , pathology , platelet , lipoprotein , cholesterol
Abstract Background Stabilin‐2 is an endocytic scavenger receptor that mediates the clearance of glycosaminoglycans, phosphatidylserine‐expressing cells, and the von Willebrand factor‐factor VIII (FVIII) complex. In a genome‐wide screening study, pathogenic loss‐of‐function variants in the human STAB2 gene associated with an increased incidence of unprovoked venous thromboembolism (VTE). However, the specific mechanism(s) by which stabilin‐2 deficiency influences the pathogenesis of VTE is unknown. Objectives The aim of this study was to assess the influence of stabilin‐2 on deep vein thrombosis (DVT) and to characterize the underlying prothrombotic phenotype of stabilin‐2 deficiency in a mouse model. Methods DVT was induced using the inferior vena cava (IVC) stenosis model in two independent cohorts (littermates and non‐littermates) of wild‐type ( Stab2 +/+ ) and stabilin‐2 ( Stab2 −/− )–deficient mice. Thrombus structure and contents were quantified by immunohistochemistry. Plasma procoagulant activity was assessed and complete blood counts were performed. Results Incidence of thrombus formation was not altered between Stab2 +/+ and Stab2 −/− mice. When thrombi were formed, Stab2 −/− mice developed significantly larger thrombi than Stab2 +/+ controls. Thrombi from Stab2 −/− mice contained significantly more leukocytes and citrullinated histone H3 than Stab2 +/+ thrombi. Stab2 −/− mice had increased FVIII activity. Circulating levels of monocytes and granulocytes were significantly elevated in Stab2 −/− mice, and Stab2 −/− mice had elevated plasma cell‐free DNA 24 hours post‐IVC stenosis compared to their Stab2 +/+ counterparts. Conclusions These data suggest that stabilin‐2 deficiency associates with a prothrombotic phenotype involving elevated levels of neutrophil extracellular trap‐releasing leukocytes coupled with endogenous procoagulant activity, resulting in larger and qualitatively distinct venous thrombi.