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Megakaryocytes use in vivo podosome‐like structures working collectively to penetrate the endothelial barrier of bone marrow sinusoids
Author(s) -
Eckly Anita,
Scandola Cyril,
Oprescu Antoine,
Michel Deborah,
Rinckel JeanYves,
Proamer Fabienne,
Hoffmann David,
Receveur Nicolas,
Léon Catherine,
Bear James E.,
Ghalloussi Dorsaf,
Harousseau Gabriel,
Bergmeier Wolfgang,
Lanza Francois,
GaitsIacovoni Frédérique,
Salle Henri,
Gachet Christian
Publication year - 2020
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/jth.15024
Subject(s) - podosome , thrombopoiesis , megakaryocyte , microbiology and biotechnology , platelet , bone marrow , endothelial stem cell , endothelium , in vivo , haematopoiesis , sinusoid , biology , cytoplasm , chemistry , cell , immunology , stem cell , in vitro , cytoskeleton , biochemistry , endocrinology
Background Blood platelets are anucleate cell fragments that prevent bleeding and minimize blood vessel injury. They are formed from the cytoplasm of megakaryocytes located in the bone marrow. For successful platelet production, megakaryocyte fragments must pass through the sinusoid endothelial barrier by a cell biology process unique to these giant cells as compared with erythrocytes and leukocytes. Currently, the mechanisms by which megakaryocytes interact and progress through the endothelial cells are not understood, resulting in a significant gap in our knowledge of platelet production. Objective The aim of this study was to investigate how megakaryocytes interact and progress through the endothelial cells of mouse bone marrow sinusoids. Methods We used a combination of fluorescence, electron, and three‐dimensional microscopy to characterize the cellular events between megakaryocytes and endothelial cells. Results We identified protrusive, F‐actin‐based podosome‐like structures, called in vivo‐MK podosomes, which initiate the formation of pores through endothelial cells. These structures present a collective and spatial organization through their interconnection via a contractile network of actomyosin, essential to regulate the endothelial openings. This ensures proper passage of megakaryocyte‐derived processes into the blood circulation to promote thrombopoiesis. Conclusion This study provides novel insight into the in vivo function of podosomes of megakaryocytes with critical importance to platelet production.