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Fibrinogen αC‐regions are not directly involved in fibrin polymerization as evidenced by a “Double‐Detroit” recombinant fibrinogen mutant and knobs‐mimic peptides
Author(s) -
Duval Cédric,
Profumo Aldo,
Aprile Anna,
Salis Annalisa,
Millo Enrico,
Damonte Gianluca,
Gauer Julia S.,
Ariëns Robert A. S.,
Rocco Mattia
Publication year - 2020
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/jth.14725
Subject(s) - fibrin , chemistry , fibrinogen , thrombin , mutant , cleavage (geology) , high performance liquid chromatography , polymerization , recombinant dna , turbidimetry , biophysics , biochemistry , microbiology and biotechnology , chromatography , platelet , polymer , biology , paleontology , organic chemistry , fracture (geology) , gene , immunology
Background Fibrin polymerization, following fibrinopeptides A and B (FpA, FpB) cleavage, relies on newly exposed α‐ and β‐chains N‐termini (GPR, GHR; A‐ , B ‐knobs, respectively) engaging preexistent a and b pockets in other fibrin(ogen) molecules' γ‐ and (B)β‐chains C‐terminal regions. A role for mostly disordered (A)α‐chains C‐terminal regions “bridging” between fibrin molecules/fibrils has been proposed. Objectives Fibrinogen Detroit is a clinically observed mutation (AαR19 → S) with nonengaging GPS A ‐knobs. By analogy, a similar Bβ‐chain mutation, BβR17 → S, should produce nonengaging GHS B ‐knobs. A homozygous “Double‐Detroit” mutant (AαR19 → S, BβR17 → S; DD‐FG) was developed: with A ‐ a and B ‐ b engagements endogenously blocked, other interactions would become apparent. Methods DD‐FG, wild‐type recombinant (WT‐FG), and human plasma (hp‐FG) fibrinogen self‐association was studied by turbidimetry coupled with fibrinopeptides release high‐performance liquid chromatography (HPLC)/mass spectrometry analyses, and by light‐scattering following size‐exclusion chromatography (SE‐HPLC). Results In contrast to WT‐FG and hp‐FG, DD‐FG produced no turbidity increase, irrespective of thrombin concentration. The SE‐HPLC profile of concentrated DD‐FG was unaffected by thrombin treatment, and light‐scattering, at lower concentration, showed no intensity and hydrodynamic radius changes. Compared with hp‐FG, both WT‐FG and DD‐FG showed no FpA cleavage difference, while ~50% FpB was not recovered. Correspondingly, SDS‐PAGE/Western‐blots revealed partial Bβ‐chain N‐terminal and Aα‐chain C‐terminal degradation. Nevertheless, ~70% DD‐FG molecules bearing (A)αC‐regions potentially able to associate were available. Higher‐concentration, nearly intact hp‐FG with 500‐fold molar excess GPRP‐NH 2 /GHRP‐NH 2 knobs‐mimics experiments confirmed these no‐association findings. Conclusions (A)αC‐regions interactions appear too weak to assist native fibrin polymerization, at least without knobs engagement. Their role in all stages should be carefully reconsidered.