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Implementation of buffy‐coat‐derived pooled platelet concentrates for internal quality control of light transmission aggregometry: a proof of concept study
Author(s) -
Prüller F.,
Rosskopf K.,
Mangge H.,
Mahla E.,
von Lewinski D.,
Weiss E. C.,
Riegler A.,
Enko D.
Publication year - 2017
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/jth.13870
Subject(s) - buffy coat , platelet , medicine , whole blood , immunology
Essentials In platelet function testing, standardized internal controls (IQC) are not commercially provided. Platelet function testing was performed daily on aliquoted pooled platelet concentrates. Pooled platelet concentrates showed stability for control purposes from Monday to Friday. Pooled platelet concentrates provide the necessary steadiness to serve as IQC material.Summary Background Standardized commercially available control material for internal quality control ( IQC ) of light transmission aggregometry ( LTA ) is still lacking. Moreover, the availability of normal blood donors to provide fresh platelets is difficult in small laboratories, where ‘volunteers’ may be in short supply. Objectives To evaluate the implementation of buffy‐coat‐derived pooled platelet concentrates ( PC s) for IQC material for LTA . Methods We used buffy‐coat‐derived pooled PC s from the blood bank as IQC material for LTA . On each weekend one PC was prepared (> 200 mL ) and aliquoted from the original storage bag on a daily basis in four baby bags (40–50 mL ), which were delivered from Monday to Friday to our laboratory. The IQC measurements of at least 85 work‐weeks (from Monday to Friday) were evaluated with this new IQC material. LTA was performed on a four‐channel Chronolog 700 Aggregometer (Chronolog Corporation, Havertown, PA , USA ) (agonists: collagen, adenosine diphosphate [ ADP ], arachidonic acid [ AA ] and thrombin receptor activator peptide‐6 [ TRAP ‐6]). Results The medians of platelet aggregation from IQC measurements with collagen, ADP and AA from Monday to Friday were 68.0–59.5, 3.0–2.0 and 51.0–50.0%, respectively, and the mean of platelet aggregation with TRAP ‐6 was 71.2–66.4%. Conclusions Buffy‐coat‐derived pooled PC s serve as a reliable and robust IQC material for LTA measurements and would be beneficial for the whole laboratory procedure and employees’ safety.