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Molecular diversity of the 5S nuclear ribosomal DNA in Campeiostachys with StHY haplome constitution
Author(s) -
Yang CaiRong,
Baum Bernard R.,
Johnson Douglas A.,
Zhang HaiQin,
Zhou YongHong
Publication year - 2020
Publication title -
journal of systematics and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.249
H-Index - 46
eISSN - 1759-6831
pISSN - 1674-4918
DOI - 10.1111/jse.12484
Subject(s) - biology , elymus , operational taxonomic unit , ribosomal dna , botany , chloroplast dna , clade , phylogenetic tree , evolutionary biology , genetics , gene , 16s ribosomal rna , poaceae
To detect the genomic constitutions and investigate the evolutionary relationships between Campeiostachys Drobov and Elymus L. species, we have cloned and analyzed 271 5S nuclear ribosomal DNA sequences from 27 accessions of these species, mostly of Chinese origin. We identified Long H 1, Short S 1, and Long Y 1 unit classes in nine Campeiostachys or Elymus species. The identification of the three orthologous unit classes was confirmed by the neighbor‐joining tree of each unit class from PAUP and the phylogeny tree of three unit classes from MrBayes. The results suggested that these Elymus species comprise StYH haplomes and should be included in Campeiostachys . The phylogeny tree showed a clear separation between the S 1 unit class and Y 1 unit class. However, Y 1 unit class sequences formed a sister clade to the S 1 unit class, implying that although the St and Y haplomes might have some affinity, they are distinct from one another. The phylogeny tree also indicated that the five species in sect. Turczaninovia ( C. dahurica var. cylindrica , C. dahurica var. dahurica , C. dahurica var. tangutorum , E. purpuraristatus , and E. dahuricus Turcz. ex Griseb. var. violeus C. P. Wang & H. L. Yang) might share a more recent common ancestor, whereas the four species in sect. Elymus ( C. nutans , E. breviaristatus (Keng) Keng ex Keng f., E. sinosubmuticus (Keng) Keng f., and E. atratus (Nevski) Hand.‐Mazz.) share a close relationship. By identifying only one type of unit class for each haplome, we propose that the 5S nuclear ribosomal DNA sequences of species within Campeiostachys might have undergone haplome‐specific concerted evolution.