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Exploratory analysis of anti‐insulin antibodies in diabetic dogs receiving recombinant human insulin
Author(s) -
Lester M.,
O'Kell A. L.
Publication year - 2020
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/jsap.13102
Subject(s) - medicine , insulin , radioimmunoassay , antibody , recombinant dna , diabetes mellitus , human insulin , endocrinology , immunology , biology , biochemistry , gene
Objectives To quantify anti‐insulin antibodies in diabetic dogs treated with recombinant human insulin and to determine if insulin dosage or duration of treatment differed between anti‐insulin antibody‐positive and ‐negative diabetic dogs. Materials and Methods Descriptive preliminary study using serum from 24 client‐owned diabetic dogs treated for a minimum of 2 weeks with recombinant human insulin, and 24 client‐owned healthy control dogs without diabetes. Sera were analysed by radioimmunoassay for anti‐insulin antibodies. The proportion of antibody positive dogs was compared between groups by Fisher's exact test. Results Four diabetic (16.6%) and no control dogs were anti‐insulin antibody positive. Clinical Significance These results indicate that treatment with recombinant human insulin may induce anti‐insulin antibodies in dogs, although this finding needs to be re‐investigated in a larger study to investigate the impact of anti‐insulin antibodies on glycaemic control.