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Oral microbiota and atherothrombotic carotid plaque vulnerability in periodontitis patients. A cross‐sectional study
Author(s) -
Brun Adrian,
Nuzzo Alexandre,
Prouvost Bastien,
Diallo Devy,
Hamdan Sandrella,
Meseguer Elena,
Guidoux Céline,
Lavallée Philippa,
Amarenco Pierre,
Lesèche Guy,
Bouchard Philippe,
Michel JeanBaptiste,
Rangé Hélène
Publication year - 2021
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12826
Subject(s) - periodontitis , medicine , myeloperoxidase , porphyromonas gingivalis , immunology , pathology , gastroenterology , inflammation
Background An increased risk of atherothrombotic vascular events has been reported in periodontitis patients. Periodontitis is associated with dysbiotic subgingival biofilms and bacteremia. Objective We hypothesized (a) that the oral microbiome is associated with the carotid microbiome and (b) that periodontitis could contribute to plaque vulnerability. The aim of this study was to determine the associations between periodontitis, the carotid microbiome, and the local innate immune response in carotid atherothrombotic plaques vulnerable to rupture. Methods In this cross‐sectional study, 45 patients admitted for carotid endarterectomy underwent a preoperative periodontal examination. The volume of intraplaque hemorrhage reflected by the hemoglobin level released in carotid‐conditioned media was considered as a criterion of carotid plaque vulnerability. Levels of antibodies against periodontal bacteria were determined in sera. The signature of the oral microbiota was assessed by microbial whole‐genome sequencing, nested PCR, and immunostaining in carotid plaque samples. Markers of neutrophil recruitment (leukotriene B4), neutrophil activation (myeloperoxidase, defensins), and cytokines were measured in carotid‐conditioned media and/or plasma. Results All patients exhibited periodontitis. One hundred and forty‐four bacterial genera were detected in the carotid microbiome. While Streptococcus was found in 84% of the carotid samples, periodontitis‐associated genera were detected in 21%. P. gingivalis DNA and gingipains were also identified in carotid samples. There were significant inverse correlations between periodontal attachment loss/serum anti‐ P. gingivalis Immunoglobulin A and cytokine inhibiting neutrophils (all P < .01). There were also significant positive correlations between lipopolysaccharides, myeloperoxidase/human neutrophil peptides1‐3, and hemoglobin levels (all P < .01). Conclusions In patients at risk of stroke, the carotid plaque microbiome was highly diverse and compatible with an oral origin. Periodontitis was significantly associated with neutrophil activation markers and plaque vulnerability to rupture.