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Potential roles of miR‐335‐5p on pathogenesis of experimental periodontitis
Author(s) -
Lian Junxiang,
Wu Xingwen,
Liu Yao,
Qiu Wei,
Zhu Xiaofang,
Wang Xiaoxuan,
Meng Shu,
Valverde Paloma,
Steffensen Bjorn,
Tu Qisheng,
Pan Jian,
Chen Jake
Publication year - 2020
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12701
Subject(s) - periodontitis , dental alveolus , inflammation , pathogenesis , immunohistochemistry , pathology , medicine , tlr4 , h&e stain , dentistry
Background and Objective Periodontitis is a prevalent oral disease responsible for tooth loss. MicroRNAs have been proven crucial in bone disorders over the past decades. Promotive effect on osteogenic activities by microRNA‐335‐5p (miR‐335‐5p) has been well demonstrated, but its role involved in the pathogenesis of periodontitis remains elusive. In this study, we established experimental periodontitis (EP) on transgenic mice overexpressing miR‐335‐5p (335‐Tg) to investigate the novel effects of miR‐335‐5p on periodontal inflammation and bone loss. Methods Experimental periodontitis was established via ligation. The expression of inflammatory and osteoclastic genes was examined by quantitative real‐time PCR (qPCR). Morphology of alveolar bone was analyzed by microcomputed tomography (μCT). Hematoxylin and eosin (H&E), tartrate‐resistant acid phosphatase (TRAP), and Toll‐like receptor 4 (TLR4) immunohistochemistry (IHC) staining were conducted for histological analysis. Results The expression of miR‐335‐5p decreased significantly in the periodontal tissues of EP. Compared to the WT‐EP group, μCT analysis showed less bone loss in the 335‐Tg‐EP group accompanying with a decreased number of TRAP‐positive osteoclasts. H&E and IHC staining exhibited attenuated inflammation and TLR4 expression in the 335‐Tg‐EP group. Furthermore, reduced expressions of IL‐1β, IL‐6, TNF‐α, and TLR4 were also detected in the 335‐Tg‐EP group. Overexpression of miR‐335‐5p in vivo weakened the periodontal bone destruction and inflammation compared with the WT‐EP group. Conclusions Our data exhibit novel roles of miR‐335‐5p in preventing bone loss and inflammation in experimental periodontitis.