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Immunohistochemical study of CD 34 and podoplanin in periodontal disease
Author(s) -
Gonçalves Patrícia Guerra Peixe,
Lourenço Salomão Israel Monteiro,
de Vasconcelos Gurgel Bruno Cesar
Publication year - 2019
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12635
Subject(s) - podoplanin , pathology , medicine , lymphangiogenesis , lymphatic system , chronic periodontitis , immunohistochemistry , gingivitis , periodontitis , lymphatic vessel , h&e stain , angiogenesis , cancer , dentistry , metastasis
Background and objective The aim of this study was to evaluate the angiogenesis and lymphangiogenesis in gingival tissue biopsy specimens of individuals with clinically healthy gingiva, chronic gingivitis, and chronic periodontitis (n = 30 per clinical condition). Material and methods Histological sections were stained using hematoxylin and eosin as well as immunohistochemically with hematopoietic progenitor cell antigen CD 34 and podoplanin ( PDPN ) antibodies to evaluate the microvascular count, area, and perimeter of blood and lymphatic vessels, respectively. Results The results revealed a correlation between the microvascular count of blood and lymphatic vessels ( P = 0.03; however, in individuals with chronic periodontitis, fewer lymphatic vessels were present than in the clinically healthy gingival tissue ( P = 0.01), which was not observed in the case of microvascular area and perimeter. Podoplanin labeling was present in the epithelium, and the intensity of labeling was positively correlated to the intensity of the inflammatory infiltrate ( P = 0.03). Conclusion In this study, we concluded that an increase in the number of blood and lymphatic vessels was not observed in bouth gingivitis and periodontitis samples. Podoplanin expression is highly associated with an increased inflammatory infiltration suggesting that PDPN might play an additional role in periodontal disease, other than solely as a lymphangiogenesis marker.