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Matrix metalloproteinase‐ 7 in periodontitis with type 2 diabetes mellitus
Author(s) -
ZeidánChuliá F.,
Yilmaz D.,
Häkkinen L.,
Könönen E.,
Neves de Oliveira B.H.,
Güncü G.,
Uitto V.J.,
Caglayan F.,
Gürsoy U. K.
Publication year - 2018
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12583
Subject(s) - matrix metalloproteinase , periodontitis , in silico , diabetes mellitus , type 2 diabetes mellitus , medicine , type 2 diabetes , in vivo , matrix (chemical analysis) , endocrinology , pathology , chemistry , biology , gene , biochemistry , genetics , chromatography
Background and Objective Elevated levels of matrix metalloproteinase‐7 ( MMP 7) have been observed in serum samples of subjects with type 2 diabetes mellitus (T2 DM ) and in gingival tissues of subjects with periodontitis. The aim of the present study was to collect in vivo and in silico evidence on the role of MMP 7 in the interplay between T2 DM and generalized periodontitis (GP). Material and Methods The extent of MMP 7 expression and localization were immunohistochemically analyzed in gingival tissues of patients with GP with T2 DM (T2 DM / GP , n = 11), systemically healthy patients with GP (n = 7), and systemically and periodontally healthy controls (n = 11). An in silico network model was built to determine the interactions between MMP 7 and T2 DM pathways. Regulation of neutrophil transmigration by MMP 7 was analyzed in a knock‐out mice model. Results In human gingival tissues, the proportion of cells with robust MMP 7 expression was elevated in patients with T2 DM / GP in comparison to controls ( P = .014). According to the in silico analysis, “hydroxyl radical” and “hydrogen peroxide” compounds were among the most central nodes of the network, and were within the shortest paths connecting “glucose” to “ MMP 7.” In MMP 7 knock‐out mice, an intense accumulation of neutrophils was observed in the gingival epithelium as compared to wild‐type mice ( P = .0001). Conclusion Elevated MMP 7 expression in gingival tissues of patients with T2 DM / GP is related to the activation of reactive oxygen species by hyperglycemia. Suppression of MMP 7 expression results in impaired neutrophil transmigration in gingiva.