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Anti‐inflammatory character of Phelligridin D modulates periodontal regeneration in l ipopolysaccharide ‐induced human periodontal ligament cells
Author(s) -
Kim J.E.,
Takanche J.S.,
Yun B.S.,
Yi H.K.
Publication year - 2018
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12570
Subject(s) - periodontal fiber , chemistry , microbiology and biotechnology , dental alveolus , kinase , cell adhesion molecule , periodontitis , western blot , inflammation , immunology , biochemistry , dentistry , biology , medicine , gene
Background and Objective Phelligridin D is a hispidin analogue from the mushroom Phellinus baumii that is widely used as a food source in East Asia. This study tested phelligridin D for the anti‐inflammatory effect and mechanism in lipopolysaccharide ( LPS) ‐induced human periodontal ligament cells ( HPDLC s). The objective of this study was to clarify whether the anti‐inflammatory function of phelligridin D affects periodontal regeneration for supporting the HPDLC s of teeth. Material and Methods Primary HPDLC s were isolated from healthy teeth and then cultured. The anti‐inflammatory function, mechanism and differentiation molecules were verified with reactive oxygen species generation and western blot analysis in LPS ‐induced HPDLC s. Results HPDLCs showed increased inflammatory molecules (intracellular adhesion molecule‐1 and vascular cell adhesion molecule‐1) and decreased osteogenic proteins (bone morphogenetic protein‐7, Osterix and runt‐related transcription factor 2) by LPS treatment. Phelligridin D decreased inflammatory molecules and increased osteogenic molecules via downregulation of the extracellular signal‐regulated kinase and c‐jun N‐terminal kinases pathway among the mitogen‐activated protein kinase, followed by blocking of nuclear factor kappa‐B translocation from cytosol to nucleus. In addition, phelligridin D showed antioxidant properties by reducing r eactive oxygen species activity. Finally, the anti‐inflammatory and antioxidant function of phelligridin D promoted the periodontal differentiation of HPDLC s. Conclusion These results suggest that phelligridin D supports teeth on the alveolar bone against outside stress, and may be used as an anti‐inflammatory compound for the prevention of periodontitis or periodontal regenerative related disease.