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Antibiotic administration alleviates the aggravating effect of orthodontic force on ligature‐induced experimental periodontitis bone loss in mice
Author(s) -
Shi J.,
Liu Z.,
Kawai T.,
Zhou Y.,
Han X.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12439
Subject(s) - ligature , medicine , periodontitis , osteoprotegerin , dentistry , molar , systemic administration , antibiotics , endocrinology , activator (genetics) , receptor , chemistry , in vivo , biology , biochemistry , microbiology and biotechnology
Background and Objectives It is recognized that orthodontic force (OF) has an aggravating effect on the progression of destructive periodontitis if periodontitis have not been well controlled. However, the underlying mechanism is not completely clear. This study was to investigate the effect of antibiotic administration on OF‐aggravated, ligature‐induced experimental periodontitis in mice. Material and Methods C57 BL /6 mice (male, 8 wk old) were divided into three groups ( n = 8). Silk ligatures ( SL ) were tied around the maxillary right (group 1) or both (groups 2 and 3) first molars on day 0, removed on day 8 and systemic antibiotics was administered through drinking water (group 3) since day 8. O F was applied on the maxillary right first molars since day 13 (groups 2 and 3). All mice were killed on day 20. Results Total oral bacteria load was significantly higher in group 2 when compared to group 1 on day 20, whereas such count was greatly reduced in group 3 when antibiotics were administered. Periodontal bone loss was significantly increased on SL side vs. control side in group 1. Periodontal bone loss was significantly increased on OF + SL side vs. SL side in group 2 ( p < 0.05) but not in group 3 when systemic antibiotics were administered. Gingival mRNA and protein expressions of receptor activator of nuclear factor kappa‐B ligand/osteoprotegerin were significantly increased on OF + SL side vs. SL side in group 2 ( p < 0.01) but not in group 3. However, comparable levels of t artrate‐resistant acid phosphatase ‐positive cell formation within periodontal space and tooth movement were observed on OF + SL side in groups 2 and 3. Conclusion Our results suggest that reduction of oral bacterial load by antibiotic administration alleviate orthodontic force‐aggravated periodontitis bone loss.