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Matrix metalloproteinase ‐8 activity in gingival crevicular fluid: development of a novel assay
Author(s) -
Mc Crudden M. T. C.,
Irwin C. R.,
El karim I.,
Linden G. J.,
Lundy F. T.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12423
Subject(s) - matrix metalloproteinase , periodontal disease , connective tissue , matrix (chemical analysis) , chemistry , basal (medicine) , clinical attachment loss , pathological , gingival and periodontal pocket , periodontitis , dentistry , medicine , pathology , biochemistry , chromatography , insulin
Background and Objective The matrix metalloproteinases ( MMP s) play a role in regulating turnover and metabolism of connective tissues in health but they have also been implicated in a wide variety of pathological conditions, including periodontal disease. MMP ‐8 has been extensively studied in periodontal health and disease using ELISA , although this technique is limited by its inability to determine enzyme activity. The aim was to develop an assay specifically to measure MMP ‐8 activity and to demonstrate its use in the analysis of gingival crevicular fluid samples. Material and Methods A specific antibody was used to coat black 96‐well microtitre plates to capture MMP‐8 selectively. The activity of bound MMP ‐8 was measured using a fluorogenic substrate. Gingival crevicular fluid samples, from healthy and periodontally diseased sites, were collected using PerioPaper strips and tested for MMP ‐8 activity. Results Significantly higher MMP ‐8 activity was demonstrated in gingival crevicular fluid from periodontally diseased sites compared with healthy sites that exhibited basal or no MMP ‐8 activity. No cross‐reactivity with other MMP s was noted. Conclusion We show, for the first time, that MMP ‐8 activity can be specifically detected and quantified in gingival crevicular fluid samples. Measurement of MMP ‐8 activity could prove to be useful in monitoring periodontal disease progression.