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Mannose‐binding lectin gene polymorphism in relation to periodontal infection
Author(s) -
Liukkonen A.,
He Q.,
Gürsoy U. K.,
Pussinen P. J.,
GröndahlYliHannuksela K.,
Liukkonen J.,
Sorsa T.,
Suominen A. L.,
Huumonen S.,
Könönen E.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12420
Subject(s) - mannan binding lectin , lectin , gene , polymorphism (computer science) , mannose , biology , genetics , microbiology and biotechnology , biochemistry , genotype
Background and Objective Mannose‐binding lectin ( MBL ) plays an important role in innate immunity. MBL deficiency is usually caused by mutations in exon 1 of the MBL structural gene ( MBL 2 ). Our aim was to investigate MBL 2 polymorphisms and their relation to salivary levels of periodontal inflammatory/tissue destruction markers and two major periodontitis‐associated bacteria. Material and Methods Salivary samples from 222 subjects were available for genotyping by pyrosequencing. The subjects between 40 and 60 years of age and having a minimum of 20 teeth were divided into three periodontal groups: 80 had generalized periodontitis, 65 had localized periodontitis and 77 were periodontitis‐free. A comparison between their MBL 2 genotypes and salivary detection rates and levels of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis as well as interleukin ‐1β, matrix metalloproteinase ‐8, and tissue inhibitor of matrix metalloproteinase ( TIMP )‐1 was performed. Results The frequencies of the MBL 2 wild‐type (A/A), heterozygote variants (A/O) and homozygote variants (O/O) were 69.4%, 26.6% and 4%, respectively. In A. actinomycetemcomitans ‐positive subjects having homozygote or heterozygote MBL 2 variants, the salivary concentrations of IL ‐1β ( p = 0.010) were elevated and those of TIMP ‐1 ( p = 0.001) were decreased. In addition their matrix metalloproteinase ‐8/ TIMP ‐1 ratio was higher ( p < 0.001) and they had more pocket teeth ( p = 0.012) than subjects negative for A. actinomycetemcomitans . Conclusion Our findings indicate that the carriage of A. actinomycetemcomitans may facilitate extended periodontal inflammation and destruction in subjects with a variant form of human MBL 2 .