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Complete physical mapping of IL 6 reveals a new marker associated with chronic periodontitis
Author(s) -
Farhat S. B.,
Souza C. M.,
Braosi A. P. R.,
Kim S. H.,
Tramontina V. A.,
Papalexiou V.,
Olandoski M.,
Mira M. T.,
Luczyszyn S. M.,
Trevilatto P. C.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12389
Subject(s) - chronic periodontitis , periodontitis , medicine , international hapmap project , single nucleotide polymorphism , dentistry , population , gastroenterology , genotype , biology , gene , genetics , environmental health
Background and Objective Interleukin‐6 ( IL ‐6) is a powerful stimulator of osteoclast differentiation and bone resorption. Production of IL ‐6 is modulated by polymorphisms, and higher levels of this cytokine are found locally in patients with chronic periodontitis. In this study we performed a modern approach – Complete physical mapping of the IL6 gene – to identify the polymorphisms associated with chronic periodontitis in a southern Brazilian population sample. Material and Methods One‐hundred and nine individuals of both genders (mean age: 41.5 ± 8.5 years) were divided into a study group (56 participants with periodontitis) and a control group (53 individuals without periodontitis). After collection and purification of DNA , nine tag single nucleotide polymorphisms ( SNP s; rs1524107, rs2069835, rs2069837, rs2069838, rs2069840, rs2069842, rs2069843, rs2069845 and rs2069849) covering the entire gene were selected according to the information available on the International HapMap Project website and evaluated using real‐time PCR . Results Differences in the distribution of the following parameters were statistically significant between study and control groups: number of teeth ( p = 0.030); probing depth ( p < 0.001); clinical attachment level ( p < 0.001); gingival index ( p < 0.001); plaque index ( p = 0.003); calculus index ( p < 0.001); and dental mobility ( p < 0.001). It was found that marker rs2069837 (located in intron 2 of IL 6 ) under G dominant was associated with protection against chronic periodontitis in a Brazilian population in the presence of clinical variables, such as visible plaque, dentist visit frequency and dental floss use, and was suggested for the first time as a marker of susceptibility to chronic periodontitis. Conclusion Complete physical mapping of IL 6 (using tag SNP s) was carried out for the first time, unveiling allele G of polymorphism rs2069837 (located in the second intron of IL 6 ) as a suggestive marker of protection against chronic periodontitis in a Brazilian population.