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Human periodontal ligament stem cells suppress T‐cell proliferation via down‐regulation of non‐classical major histocompatibility complex‐like glycoprotein CD 1b on dendritic cells
Author(s) -
Shin C.,
Kim M.,
Han J.A.,
Choi B.,
Hwang D.,
Do Y.,
Yun J.H.
Publication year - 2017
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12378
Subject(s) - periodontal ligament stem cells , mesenchymal stem cell , cd146 , stem cell , cd80 , immune system , dendritic cell , microbiology and biotechnology , immunology , antigen presenting cell , porphyromonas gingivalis , t cell , chemistry , biology , periodontitis , cd40 , cytotoxic t cell , medicine , in vitro , alkaline phosphatase , cd34 , biochemistry , enzyme
Background and Objective Periodontal ligament stem cells ( PDLSC s) from the periodontal ligament tissue were recently identified as mesenchymal stem cells ( MSC s). The capabilities of PDLSC s in periodontal tissue or bone regeneration have been reported, but their immunomodulatory role in T‐cell immune responses via dendritic cells ( DC s), known as the most potent antigen‐presenting cell, has not been studied. The aim of this study is to understand the immunological function of homogeneous human STRO ‐1 + CD 146 + PDLSC s in DC ‐mediated T‐cell immune responses to modulate the periodontal disease process. Material and Methods We utilized highly purified (> 95%) human STRO ‐1 + CD 146 + PDLSC s and human bone marrow mesenchymal stem cells ( BMSC s). Each stem cell was co‐cultured with human monocyte‐derived DC s in the presence of lipopolysaccharide isolated from Porphyromonas gingivalis , a major pathogenic bacterium responsible for periodontal disease, in vitro to examine the immunological effect of each stem cell on DC s and DC ‐mediated T‐cell proliferation. Results We discovered that STRO ‐1 + CD 146 + PDLSC s, as well as BMSC s, significantly decreased the level of non‐classical major histocompatibility complex glycoprotein CD 1b on DC s, resulting in defective T‐cell proliferation, whereas most human leukocyte antigens and the co‐stimulatory molecules CD 80 and CD 86 in/on DC s were not significantly affected by the presence of BMSC s or STRO ‐1 + CD 146 + PDLSC s. Conclusions This study unveiled an immunomodulatory role of STRO ‐1 + CD 146 + PDLSC s in negatively regulating DC ‐mediated T‐cell immune responses, demonstrating their potential to be utilized in promising new stem cell therapies.