Premium
Periodontal tissue engineering by nano beta‐tricalcium phosphate scaffold and fibroblast growth factor‐2 in one‐wall infrabony defects of dogs
Author(s) -
Ogawa K.,
Miyaji H.,
Kato A.,
Kosen Y.,
Momose T.,
Yoshida T.,
Nishida E.,
Miyata S.,
Murakami S.,
Takita H.,
Fugetsu B.,
Sugaya T.,
Kawanami M.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12352
Subject(s) - scaffold , fibroblast , biomedical engineering , basic fibroblast growth factor , periodontal fiber , materials science , fibroblast growth factor , tissue engineering , chemistry , growth factor , dentistry , medicine , in vitro , biochemistry , receptor
Background and Objective Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta‐tricalcium phosphate (β‐ TCP ) nanoparticles. Fibroblast growth factor‐2 ( FGF ‐2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects, on bioactivity, of a nano‐β‐ TCP /collagen scaffold loaded with FGF ‐2, particularly on periodontal tissue wound healing. Material and Methods Beta‐tricalcium phosphate was pulverized into nanosize particles (84 nm) and was then dispersed. A nano‐β‐ TCP scaffold was prepared by coating the surface of a collagen scaffold with a nanosize β‐ TCP dispersion. Scaffolds were characterized using scanning electron microscopy, compressive testing, cell seeding and rat subcutaneous implant testing. Then, nano‐β‐ TCP scaffold, nano‐β‐ TCP scaffold loaded with FGF ‐2 and noncoated collagen scaffold were implanted into a dog one‐wall infrabony defect model. Histological observations were made at 10 d and 4 wk postsurgery. Results Scanning electron microscopy images show that TCP nanoparticles were attached to collagen fibers. The nano‐β‐ TCP scaffold showed higher compressive strength and cytocompatibility compared with the noncoated collagen scaffold. Rat subcutaneous implant tests showed that the DNA contents of infiltrating cells in the nano‐β‐ TCP scaffold and the FGF‐ 2‐loaded scaffold were approximately 2.8‐fold and 3.7‐fold greater, respectively, than in the collagen scaffold. Histological samples from the periodontal defect model showed about five‐fold greater periodontal tissue repair following implantation of the nano‐β‐ TCP scaffold loaded with FGF ‐2 compared with the collagen scaffold. Conclusion The β‐ TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano‐β‐ TCP scaffolds containing FGF ‐2 are anticipated for use in periodontal tissue engineering.