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Effect of low‐level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5‐fluorouracil chemotherapy
Author(s) -
Theodoro L. H.,
Longo M.,
Ervolino E.,
Duque C.,
FerroAlves M. L.,
Assem N. Z.,
Louzada L. M.,
Garcia V. G.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12347
Subject(s) - medicine , prevotella intermedia , aggregatibacter actinomycetemcomitans , periodontitis , adjuvant , osteoprotegerin , chronic periodontitis , porphyromonas gingivalis , dental alveolus , fusobacterium nucleatum , gastroenterology , dentistry , receptor , activator (genetics)
Background and Objective This study aimed to assess the effect of multiple sessions of a low‐level laser therapy ( LLLT ) adjuvant to scaling and root planing ( SRP ) on the treatment of experimental periodontitis ( EP ) in rats treated with 5‐fluorouracil (5‐ FU ). Material and Methods A total of 120 rats were divided into five groups: no treatment ( NT ); treatment with 5‐ FU (60 and 40 mg/kg) and no local periodontal treatment (5FU); treatment with 5‐ FU and SRP (5FU‐SRP) ; treatment with 5‐ FU , SRP and one LLLT session (660 nm; 0.035 W; 4.2 J; 120 s) (5 FU ‐ SRP ‐1LLLT); and treatment with 5‐ FU , SRP and four LLLT sessions (0, 24, 48 and 72 h) (5 FU ‐ SRP ‐4LLLT). EP was induced in the mandibular molars through ligature placement. The alveolar bone loss ( ABL ) area in the furcation region was analysed histometrically. TRAP , proliferating cell nuclear antigen, RANKL , osteoprotegerin and activated caspase‐3 patterns were analysed by immunolabeling. Prostaglandin E2 was quantified using an ELISA , and tumour necrosis factor α and interleukin‐6 were assessed using the multiplex method. The prevalence rates of Aggregatibacter actinomycetemcomitans , Porphyromonas gingivalis , Prevotella nigrescens , Prevotella intermedia and Fusobacterium nucleatum were assessed using the PCR method. The data were subjected to statistical analysis (α = 5%). Results 5 FU , 5 FU ‐ SRP and 5 FU ‐ SRP ‐1 LLLT treatment groups showed higher ABL compared with the NT group ( p < 0.05), whereas the 5 FU ‐ SRP ‐4 LLLT group showed lower ABL compared with the 5 FU group on day 7 and decreased RANKL immunolabeling ( p < 0.05). Conclusion Treatment with 5‐ FU worsened EP , and multiple LLLT sessions adjuvant to SRP seemed to improve periodontitis in rats subjected to 5‐ FU chemotherapy.