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Cytokine (interleukin‐1beta) and MMP levels in gingival crevicular fluid after use of platelet‐rich fibrin or connective tissue graft in the treatment of localized gingival recessions
Author(s) -
Eren G.,
Tervahartiala T.,
Sorsa T.,
Atilla G.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12325
Subject(s) - medicine , platelet rich fibrin , fibrin , connective tissue , gingival recession , dentistry , pathology , immunology
Background and Objective The objective of this study was to evaluate the levels of MMP ‐8, MMP‐9, TIMP ‐1 and interleukin‐1beta ( IL ‐1β) in gingival crevicular fluid during the early and late stages of healing in gingival recession sites treated with coronally advanced flap plus platelet‐rich fibrin ( CAF + PRF ) compared with CAF plus connective tissue graft ( CAF + CTG ). Material and Methods Twenty‐four nonsmoking patients with Miller Class I or Class II localized gingival recession defects in bilateral sites received treatment with either CAF + PRF ( PRF group) or CAF + CTG ( CTG group). Gingival crevicular fluid samples were collected at baseline and at 10 d and 1 mo, 3 mo and 6 mo after surgery. The levels of MMP ‐8, MMP‐9, TIMP ‐1 and IL ‐1β in gingival crevicular fluid were measured using a time‐resolved immunofluorometric assay and ELISAs. Results Gingival crevicular fluid levels of IL ‐1β were significantly elevated in the CTG group at 10 d compared with baseline ( p < 0.05). At 10 d after surgery, the levels of TIMP ‐1 in gingival crevicular fluid showed a significant decrease in the CTG group compared with the PRF group ( p < 0.05). The levels of IL ‐1β and MMP ‐8 in gingival crevicular fluid were significantly lower in the PRF group than in the CTG group at 10 d ( p < 0.05). No significant differences were found in all clinical and biochemical parameters at 1, 3, and 6 mo between study groups ( p > 0.05). Conclusion Root coverage with CAF + PRF has a significant effect on increasing gingival crevicular fluid TIMP ‐1 levels and suppressing gingival crevicular fluid MMP ‐8 and IL ‐β levels at 10 d. Gingival crevicular fluid levels of MMP ‐8, MMP‐9, TIMP ‐1 and IL ‐1β did not seem to be affected by the technique at later phases of wound healing.