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α‐tocopherol decreases interleukin‐1β and ‐6 and increases human β‐defensin‐1 and ‐2 secretion in human gingival fibroblasts stimulated with Porphyromonas gingivalis lipopolysaccharide
Author(s) -
Derradjia A.,
Alanazi H.,
Park H. J.,
Djeribi R.,
Semlali A.,
Rouabhia M.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12308
Subject(s) - porphyromonas gingivalis , fibroblast , lipopolysaccharide , secretion , cytokine , interleukin , inflammation , wound healing , chemistry , interleukin 8 , periodontitis , immunology , medicine , endocrinology , biology , in vitro , biochemistry
Background and Objective Periodontitis, a disease associated with chronic inflammation, results in significant destruction of periodontal tissues. Uncontrolled, periodontal disease negatively affects general patient health. We sought to evaluate the effect of α‐tocopherol on gingival fibroblast behavior following exposure to Porphyromonas gingivalis lipopolysaccharide ( LPS ). Material and Methods Primary human gingival fibroblasts were cultured for 24 and 48 h with α‐tocopherol at various concentrations (0, 50, 100 and 200 μ m ) in the presence or absence of 1 μg/ mL of LPS . At the end of each time point, cell adhesion and growth were evaluated by means of optical microscope observations and MTT assay. The secretion levels of cytokines interleukin ( IL) ‐1β and IL ‐6 and human β‐defensins 1 and 2 were measured by specific enzyme‐linked immunosorbent assay. Finally, an in vitro scratch wound assay was performed to investigate the effect of α‐tocopherol on fibroblast migration. Results α‐tocopherol alone had no adverse effect on cell adhesion and morphology. Fibroblast proliferation increased in the presence of α‐tocopherol with and without LPS . α‐tocopherol alone had no effect on inflammatory cytokine ( IL ‐1β and IL ‐6) secretion. Interestingly, following cell exposure to P. gingivalis LPS , α‐tocopherol significantly ( p < 0.01) decreased the secretion of these two cytokines and increased human β‐defensin ‐1 and ‐2 secretion. Finally, α‐tocopherol increased the healing rate of the gingival fibroblasts from 12 h up to 48 h. Conclusion These results suggest that α‐tocopherol may play an active role in countering the damaging effect of LPS by reducing inflammatory cytokines, increasing β‐defensins and promoting fibroblast growth, migration and wound healing.