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Characterization of progenitor cells and stem cells from the periodontal ligament tissue derived from a single person
Author(s) -
Prateeptongkum E.,
Klingelhöffer C.,
Müller S.,
Ettl T.,
Morsczeck C.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12306
Subject(s) - periodontal ligament stem cells , progenitor cell , microbiology and biotechnology , mesenchymal stem cell , stem cell , periodontal fiber , alkaline phosphatase , chemistry , dental follicle , biology , dentistry , medicine , biochemistry , enzyme
Background Periodontal ligament progenitor cells (PDLPs) and PDL stem cells ( PDLSC s) are progenitor and stem cells that were isolated from PDL tissues using the outgrowth and single cell isolation methods respectively. The differences between PDLP s and PDLSC s characteristics could be observed from previous studies. However, these cells were obtained from different patients. This study was the first report to compare the characterization of PDLP s and PDLSC s from the same person. Material and Methods The characterization of PDLP s and PDLSC s includes flow cytometry analysis, cell proliferation assay and the assessment of the colony‐forming unit fibroblast. The osteogenic differentiation was evaluated by alkaline phosphatase activity, biomineralization (alizarin red staining) and gene expression of osteogenic markers. The adipogenic differentiation was examined by Oil Red O staining and adipocyte‐related gene expression. Results Mesenchymal stem cell marker expression and colony‐forming unit fibroblast analysis of PDLP s and PDLSC s were similar. However, PDLSC s grew faster than PDLP s on days 3 and 5 of the cell proliferation assay. Both PDLP s and PDLSC s could differentiate into osteoblast and adipocyte‐like cells. However, the mineralization of PDLSC s was stronger than that of PDLP s. Conclusions The characteristics of undifferentiated PDL cells in our study were not significantly impacted by the isolation method. We assumed that both PDLP s and PDLSC s are valuable cell sources for periodontal regeneration. However, PDLSC s have a possible advantage for the regeneration of alveolar bone.