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Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue
Author(s) -
Cantley M. D.,
Dharmapatni A. A. S. S. K.,
Algate K.,
Crotti T. N.,
Bartold P. M.,
Haynes D. R.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12290
Subject(s) - periodontitis , chronic periodontitis , medicine , histone deacetylase , inflammation , immunohistochemistry , tumor necrosis factor alpha , pathology , cancer research , immunology , histone , biology , gene , biochemistry
Background and Objective Histone deacetylase inhibitors ( HDAC i) are being considered to treat chronic inflammatory diseases at low doses. Currently HDAC i that are more specific are being developed to target particular HDAC s; therefore, this study aimed to determine levels and distribution of class I and II HDAC in human gingival samples obtained from patients with chronic periodontitis. Material and Methods Gingival biopsies were obtained from patients with and without (mild inflammation, no bone loss) periodontitis. Total RNA was isolated for real‐time quantitative polymerase chain reaction to determine expression of HDACs 1–10. Immunohistochemistry was used to determine protein distribution of HDAC s 1, 5, 8 and 9. Factor VIII , CD 3 and tartrate resistant acid phosphatase (TRAP) were detected in serial sections to identify blood vessels, lymphocytes, pre‐osteoclasts and osteoclasts cells respectively. Tumour necrosis factor α (TNF‐α) expression was also assessed. Results mRNA for HDAC 1, 5, 8 and 9 were significantly upregulated in chronic periodontitis gingival tissues compared to non‐periodontitis samples ( p < 0.05). Significantly higher HDAC 1 protein expression was observed in chronic periodontitis samples ( p < 0.05), and was associated with CD 3, TRAP and TNF‐α‐positive cells. HDAC 1, 5, 8 and 9 were expressed strongly by the factor VIII ‐positive microvasculature in the chronic periodontitis gingival tissues. Conclusions HDAC 1, 5, 8 and 9 expression was higher in gingival tissues from patients with chronic periodontitis compared to non‐periodontitis samples. Results suggest that these HDAC s could therefore be targeted with specific acting HDAC i.